Primary Virus-Cell Interactions in the Immuno-fluorescence Assay of Venezuelan Equine Encephalomyelitis Virus

Primary virus-cell interactions in the immunofluorescence assay of Venezuelan equine encephalomyelitis virus.

The conditions under which Venezuelan equine encephalomyelitis (VEE) virus attached to host cells markedly influenced the assay of virus by the fluorescent cell-counting technique. When virus inoculum was centrifuged onto McCoy cell monolayers, approximately 97% of virus was attached to cells within 10 min, in contrast to 34% after stationary incubation at 35 C for 2 hr. Maximal binding of viru...

[Sedimentation characteristics of Venezuelan equine encephalomyelitis virus].

Morphogenesis of Venezuelan equine encephalomyelitis virus.

Morphogenesis of Venezuelan equine encephalomyelitis virus was studied by means of electron microscopy. Virus-specific structures (factories, viroplasts) were found at early stages of infection; these structures were composed of fibrillar and cylindrical formations, aggregates of ribosomes, and viral nucleoids. The latter emerged from fibrillar and cylindrical structures. Aggregates of viral nu...

Cell surface antigen induced by venezuelan equine encephalomyelitis virus.

By immunofluorescence staining, a specific surface antigen induced by Venezuelan equine encephalomyelitis virus was detected on L-929 cells. Formation of the antigen was independent of viral ribonucleic acid synthesis.

A slit sampler for collecting T-3 bacteriophage and Venezuelan equine encephalomyelitis virus. II. Studies with Venezuelan equine encephalomyelitis virus.

The virus employed was the fourth mouse brain passage of a strain of VEE virus recovered from a human infection in Colombia. The virus was suspended in phosphate buffer soluton (PBS),' pH 7.5, containing 2 per cent normal rabbit serum (NRS) to which antifoam A2 had been added to facilitate aerosolization. The virus suspension was aerosolized with a Collison3 generator in a modified Henderson4 a...